14 lines
631 B
Plaintext
14 lines
631 B
Plaintext
Optimise combinations of primers and minimise the formation of dimers in
|
|
multiplexed PCR.
|
|
Primer Pooler can:
|
|
* Check through each proposed pool for combinations that are likely to form
|
|
dimers
|
|
* Automatically move prospective amplicons between proposed pools to reduce
|
|
dimer formation
|
|
* Automatically search the genome sequence to find which amplicons overlap, and
|
|
place their corresponding primers in separate pools
|
|
* Optionally keep pool sizes within a specified range
|
|
* Handle thousands of primers without being slow (useful for high-throughput
|
|
sequencing applications)
|
|
* Do all of the above with degenerate primers too.
|